Application

ELISA.

Western blotting: (The antigen should be separated in SDS-PAGE under reducing conditions

and does not react with non-reduced sample.): 1:5,000.

Immunohistochemistry/Immunofluorescence: 1:1,000.

Optimal working dilutions must be determined by the end user.

Research Sub CategoryIntegrins

Research CategoryCell Structure

Detect Integrin α5 using this Anti-Integrin α5 Antibody validated for use in ELISA, WB, IH.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Recombinant fragment of mouse alpha5 integrin (Holers et al. 1989) corresponding to amino acid residues 655-880 of the human protein (Argraves 1987). This region of the molecule is extracellular and is included in the heavy chain (120 kDa).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Whole rabbit serum. Liquid, with 0.02% sodium azide.

Specificity

Specific for alpha5 integrin as determined by Western blotting.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.